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Creators/Authors contains: "Andrew Goodenough, Taylor Hatcher"

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  1. (, 70th American Society for Mass Spectrometry (ASMS) Conference, Minneapolis, MN)
    The study of the bee lipidome provides valuable information about bees’ metabolic adaptation to changes in climate. Mass spectrometry imaging is an informative technique that allows for visualization of spatial distributions of many hundreds of molecules, including lipids, from a single sample. Shotgun lipidomics is another useful technique that allows for rapid analysis of a complex sample, enabling quantitation and confident identification of lipid classes. Much is unknown about the spatial distribution, differences between species, etc. of lipids in bees. Here, we report a UV-MALDESI imaging-Orbitrap MS method for bee sections to explore the lipidome of two species: Bombus impatiens and Megachile rotundata. We show the distribution and relative quantitation of multiple lipids, and their differences across bee species. 
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